Induction of C/EBP homologous protein-mediated apoptosis and autophagy by licochalcone A in non-small cell lung cancer cells.
Identifieur interne : 000D69 ( Main/Exploration ); précédent : 000D68; suivant : 000D70Induction of C/EBP homologous protein-mediated apoptosis and autophagy by licochalcone A in non-small cell lung cancer cells.
Auteurs : Zheng-Hai Tang [République populaire de Chine] ; Xin Chen [République populaire de Chine] ; Zhao-Yu Wang [République populaire de Chine] ; Ke Chai [République populaire de Chine] ; Ya-Fang Wang [République populaire de Chine] ; Xiao-Huang Xu [République populaire de Chine] ; Xiao-Wen Wang [République populaire de Chine] ; Jia-Hong Lu [République populaire de Chine] ; Yi-Tao Wang [République populaire de Chine] ; Xiu-Ping Chen [République populaire de Chine] ; Jin-Jian Lu [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2016.
Descripteurs français
- KwdFr :
- Antinéoplasiques d'origine végétale (pharmacologie), Apoptose (), Autophagie (), Carcinome pulmonaire non à petites cellules (anatomopathologie), Carcinome pulmonaire non à petites cellules (métabolisme), Carcinome pulmonaire non à petites cellules (traitement médicamenteux), Cellules A549, Chalcones (pharmacologie), Facteur de transcription CHOP (antagonistes et inhibiteurs), Facteur de transcription CHOP (génétique), Facteur de transcription CHOP (métabolisme), Glycyrrhiza uralensis (), Humains, L-Lactate dehydrogenase (métabolisme), Lignée cellulaire tumorale, Médicaments issus de plantes chinoises (pharmacologie), Protéines associées aux microtubules (métabolisme), Survie cellulaire (), Techniques de knock-down de gènes, Tumeurs du poumon (anatomopathologie), Tumeurs du poumon (métabolisme), Tumeurs du poumon (traitement médicamenteux).
- MESH :
- anatomopathologie : Carcinome pulmonaire non à petites cellules, Tumeurs du poumon.
- antagonistes et inhibiteurs : Facteur de transcription CHOP.
- génétique : Facteur de transcription CHOP.
- métabolisme : Carcinome pulmonaire non à petites cellules, Facteur de transcription CHOP, L-Lactate dehydrogenase, Protéines associées aux microtubules, Tumeurs du poumon.
- pharmacologie : Antinéoplasiques d'origine végétale, Chalcones, Médicaments issus de plantes chinoises.
- traitement médicamenteux : Carcinome pulmonaire non à petites cellules, Tumeurs du poumon.
- Apoptose, Autophagie, Cellules A549, Glycyrrhiza uralensis, Humains, Lignée cellulaire tumorale, Survie cellulaire, Techniques de knock-down de gènes.
English descriptors
- KwdEn :
- A549 Cells, Antineoplastic Agents, Phytogenic (pharmacology), Apoptosis (drug effects), Autophagy (drug effects), Carcinoma, Non-Small-Cell Lung (drug therapy), Carcinoma, Non-Small-Cell Lung (metabolism), Carcinoma, Non-Small-Cell Lung (pathology), Cell Line, Tumor, Cell Survival (drug effects), Chalcones (pharmacology), Drugs, Chinese Herbal (pharmacology), Gene Knockdown Techniques, Glycyrrhiza uralensis (chemistry), Humans, L-Lactate Dehydrogenase (metabolism), Lung Neoplasms (drug therapy), Lung Neoplasms (metabolism), Lung Neoplasms (pathology), Microtubule-Associated Proteins (metabolism), Transcription Factor CHOP (antagonists & inhibitors), Transcription Factor CHOP (genetics), Transcription Factor CHOP (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Transcription Factor CHOP.
- chemical , genetics : Transcription Factor CHOP.
- chemical , metabolism : L-Lactate Dehydrogenase, Microtubule-Associated Proteins, Transcription Factor CHOP.
- chemical , pharmacology : Antineoplastic Agents, Phytogenic, Chalcones, Drugs, Chinese Herbal.
- chemistry : Glycyrrhiza uralensis.
- drug effects : Apoptosis, Autophagy, Cell Survival.
- drug therapy : Carcinoma, Non-Small-Cell Lung, Lung Neoplasms.
- metabolism : Carcinoma, Non-Small-Cell Lung, Lung Neoplasms.
- pathology : Carcinoma, Non-Small-Cell Lung, Lung Neoplasms.
- A549 Cells, Cell Line, Tumor, Gene Knockdown Techniques, Humans.
Abstract
Licochalcone A (LCA), a flavonoid isolated from the famous Chinese medicinal herb Glycyrrhiza uralensis Fisch, presents obvious anti-cancer effects. In this study, the anti-cancer effects and potential mechanisms of LCA in non-small cell lung cancer (NSCLC) cells were studied. LCA decreased cell viability, increased lactate dehydrogenase release, and induced apoptosis in a concentration-dependent manner in NSCLC cells while not in human embryonic lung fibroblast cells. The expression of phosphatidylethanolamine-modified microtubule-associated protein light-chain 3 (LC3-II) and formation of GFP-LC3 punta, two autophagic markers, were increased after treatment with LCA. LCA-induced LC3-II expression was increased when combined with chloroquine (CQ), while knock-down of autophagy related protein (ATG) 7 or ATG5 reversed LCA-induced LC3-II expression and GFP-LC3 punta formation, suggesting that LCA induced autophagy in NSCLC cells. Inhibition of autophagy could not reverse the LCA-induced cell viability decrease and apoptosis. In addition, LCA increased the expression of endoplasmic reticulum stress related proteins, such as binding immunoglobulin protein and C/EBP homologous protein (CHOP). Knock-down of CHOP reversed LCA-induced cell viability decrease, apoptosis, and autophagy. Taken together, LCA-induced autophagic effect is an accompanied phenomenon in NSCLC cells, and CHOP is critical for LCA-induced cell viability decrease, apoptosis, and autophagy.
DOI: 10.1038/srep26241
PubMed: 27184816
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000207
- to stream PubMed, to step Curation: 000207
- to stream PubMed, to step Checkpoint: 000204
- to stream Ncbi, to step Merge: 000340
- to stream Ncbi, to step Curation: 000340
- to stream Ncbi, to step Checkpoint: 000340
- to stream Main, to step Merge: 000D70
- to stream Main, to step Curation: 000D69
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Induction of C/EBP homologous protein-mediated apoptosis and autophagy by licochalcone A in non-small cell lung cancer cells.</title><author><name sortKey="Tang, Zheng Hai" sort="Tang, Zheng Hai" uniqKey="Tang Z" first="Zheng-Hai" last="Tang">Zheng-Hai Tang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Zhao Yu" sort="Wang, Zhao Yu" uniqKey="Wang Z" first="Zhao-Yu" last="Wang">Zhao-Yu Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Chai, Ke" sort="Chai, Ke" uniqKey="Chai K" first="Ke" last="Chai">Ke Chai</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Ya Fang" sort="Wang, Ya Fang" uniqKey="Wang Y" first="Ya-Fang" last="Wang">Ya-Fang Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiao Huang" sort="Xu, Xiao Huang" uniqKey="Xu X" first="Xiao-Huang" last="Xu">Xiao-Huang Xu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiao Wen" sort="Wang, Xiao Wen" uniqKey="Wang X" first="Xiao-Wen" last="Wang">Xiao-Wen Wang</name><affiliation wicri:level="3"><nlm:affiliation>Medical Center, Yuquan Hospital, Tsinghua University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Medical Center, Yuquan Hospital, Tsinghua University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lu, Jia Hong" sort="Lu, Jia Hong" uniqKey="Lu J" first="Jia-Hong" last="Lu">Jia-Hong Lu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yi Tao" sort="Wang, Yi Tao" uniqKey="Wang Y" first="Yi-Tao" last="Wang">Yi-Tao Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xiu Ping" sort="Chen, Xiu Ping" uniqKey="Chen X" first="Xiu-Ping" last="Chen">Xiu-Ping Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Jin Jian" sort="Lu, Jin Jian" uniqKey="Lu J" first="Jin-Jian" last="Lu">Jin-Jian Lu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27184816</idno><idno type="pmid">27184816</idno><idno type="doi">10.1038/srep26241</idno><idno type="wicri:Area/PubMed/Corpus">000207</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000207</idno><idno type="wicri:Area/PubMed/Curation">000207</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000207</idno><idno type="wicri:Area/PubMed/Checkpoint">000204</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000204</idno><idno type="wicri:Area/Ncbi/Merge">000340</idno><idno type="wicri:Area/Ncbi/Curation">000340</idno><idno type="wicri:Area/Ncbi/Checkpoint">000340</idno><idno type="wicri:Area/Main/Merge">000D70</idno><idno type="wicri:Area/Main/Curation">000D69</idno><idno type="wicri:Area/Main/Exploration">000D69</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Induction of C/EBP homologous protein-mediated apoptosis and autophagy by licochalcone A in non-small cell lung cancer cells.</title><author><name sortKey="Tang, Zheng Hai" sort="Tang, Zheng Hai" uniqKey="Tang Z" first="Zheng-Hai" last="Tang">Zheng-Hai Tang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Zhao Yu" sort="Wang, Zhao Yu" uniqKey="Wang Z" first="Zhao-Yu" last="Wang">Zhao-Yu Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Chai, Ke" sort="Chai, Ke" uniqKey="Chai K" first="Ke" last="Chai">Ke Chai</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Ya Fang" sort="Wang, Ya Fang" uniqKey="Wang Y" first="Ya-Fang" last="Wang">Ya-Fang Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiao Huang" sort="Xu, Xiao Huang" uniqKey="Xu X" first="Xiao-Huang" last="Xu">Xiao-Huang Xu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiao Wen" sort="Wang, Xiao Wen" uniqKey="Wang X" first="Xiao-Wen" last="Wang">Xiao-Wen Wang</name><affiliation wicri:level="3"><nlm:affiliation>Medical Center, Yuquan Hospital, Tsinghua University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Medical Center, Yuquan Hospital, Tsinghua University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lu, Jia Hong" sort="Lu, Jia Hong" uniqKey="Lu J" first="Jia-Hong" last="Lu">Jia-Hong Lu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yi Tao" sort="Wang, Yi Tao" uniqKey="Wang Y" first="Yi-Tao" last="Wang">Yi-Tao Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xiu Ping" sort="Chen, Xiu Ping" uniqKey="Chen X" first="Xiu-Ping" last="Chen">Xiu-Ping Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Jin Jian" sort="Lu, Jin Jian" uniqKey="Lu J" first="Jin-Jian" last="Lu">Jin-Jian Lu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao</wicri:regionArea><wicri:noRegion>Macao</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A549 Cells</term><term>Antineoplastic Agents, Phytogenic (pharmacology)</term><term>Apoptosis (drug effects)</term><term>Autophagy (drug effects)</term><term>Carcinoma, Non-Small-Cell Lung (drug therapy)</term><term>Carcinoma, Non-Small-Cell Lung (metabolism)</term><term>Carcinoma, Non-Small-Cell Lung (pathology)</term><term>Cell Line, Tumor</term><term>Cell Survival (drug effects)</term><term>Chalcones (pharmacology)</term><term>Drugs, Chinese Herbal (pharmacology)</term><term>Gene Knockdown Techniques</term><term>Glycyrrhiza uralensis (chemistry)</term><term>Humans</term><term>L-Lactate Dehydrogenase (metabolism)</term><term>Lung Neoplasms (drug therapy)</term><term>Lung Neoplasms (metabolism)</term><term>Lung Neoplasms (pathology)</term><term>Microtubule-Associated Proteins (metabolism)</term><term>Transcription Factor CHOP (antagonists & inhibitors)</term><term>Transcription Factor CHOP (genetics)</term><term>Transcription Factor CHOP (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antinéoplasiques d'origine végétale (pharmacologie)</term><term>Apoptose ()</term><term>Autophagie ()</term><term>Carcinome pulmonaire non à petites cellules (anatomopathologie)</term><term>Carcinome pulmonaire non à petites cellules (métabolisme)</term><term>Carcinome pulmonaire non à petites cellules (traitement médicamenteux)</term><term>Cellules A549</term><term>Chalcones (pharmacologie)</term><term>Facteur de transcription CHOP (antagonistes et inhibiteurs)</term><term>Facteur de transcription CHOP (génétique)</term><term>Facteur de transcription CHOP (métabolisme)</term><term>Glycyrrhiza uralensis ()</term><term>Humains</term><term>L-Lactate dehydrogenase (métabolisme)</term><term>Lignée cellulaire tumorale</term><term>Médicaments issus de plantes chinoises (pharmacologie)</term><term>Protéines associées aux microtubules (métabolisme)</term><term>Survie cellulaire ()</term><term>Techniques de knock-down de gènes</term><term>Tumeurs du poumon (anatomopathologie)</term><term>Tumeurs du poumon (métabolisme)</term><term>Tumeurs du poumon (traitement médicamenteux)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Transcription Factor CHOP</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transcription Factor CHOP</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>L-Lactate Dehydrogenase</term><term>Microtubule-Associated Proteins</term><term>Transcription Factor CHOP</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antineoplastic Agents, Phytogenic</term><term>Chalcones</term><term>Drugs, Chinese Herbal</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Carcinome pulmonaire non à petites cellules</term><term>Tumeurs du poumon</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Facteur de transcription CHOP</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Glycyrrhiza uralensis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Autophagy</term><term>Cell Survival</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Carcinoma, Non-Small-Cell Lung</term><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteur de transcription CHOP</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Carcinoma, Non-Small-Cell Lung</term><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carcinome pulmonaire non à petites cellules</term><term>Facteur de transcription CHOP</term><term>L-Lactate dehydrogenase</term><term>Protéines associées aux microtubules</term><term>Tumeurs du poumon</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Carcinoma, Non-Small-Cell Lung</term><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antinéoplasiques d'origine végétale</term><term>Chalcones</term><term>Médicaments issus de plantes chinoises</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Carcinome pulmonaire non à petites cellules</term><term>Tumeurs du poumon</term></keywords><keywords scheme="MESH" xml:lang="en"><term>A549 Cells</term><term>Cell Line, Tumor</term><term>Gene Knockdown Techniques</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Apoptose</term><term>Autophagie</term><term>Cellules A549</term><term>Glycyrrhiza uralensis</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Survie cellulaire</term><term>Techniques de knock-down de gènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Licochalcone A (LCA), a flavonoid isolated from the famous Chinese medicinal herb Glycyrrhiza uralensis Fisch, presents obvious anti-cancer effects. In this study, the anti-cancer effects and potential mechanisms of LCA in non-small cell lung cancer (NSCLC) cells were studied. LCA decreased cell viability, increased lactate dehydrogenase release, and induced apoptosis in a concentration-dependent manner in NSCLC cells while not in human embryonic lung fibroblast cells. The expression of phosphatidylethanolamine-modified microtubule-associated protein light-chain 3 (LC3-II) and formation of GFP-LC3 punta, two autophagic markers, were increased after treatment with LCA. LCA-induced LC3-II expression was increased when combined with chloroquine (CQ), while knock-down of autophagy related protein (ATG) 7 or ATG5 reversed LCA-induced LC3-II expression and GFP-LC3 punta formation, suggesting that LCA induced autophagy in NSCLC cells. Inhibition of autophagy could not reverse the LCA-induced cell viability decrease and apoptosis. In addition, LCA increased the expression of endoplasmic reticulum stress related proteins, such as binding immunoglobulin protein and C/EBP homologous protein (CHOP). Knock-down of CHOP reversed LCA-induced cell viability decrease, apoptosis, and autophagy. Taken together, LCA-induced autophagic effect is an accompanied phenomenon in NSCLC cells, and CHOP is critical for LCA-induced cell viability decrease, apoptosis, and autophagy.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Tang, Zheng Hai" sort="Tang, Zheng Hai" uniqKey="Tang Z" first="Zheng-Hai" last="Tang">Zheng-Hai Tang</name></noRegion><name sortKey="Chai, Ke" sort="Chai, Ke" uniqKey="Chai K" first="Ke" last="Chai">Ke Chai</name><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name><name sortKey="Chen, Xiu Ping" sort="Chen, Xiu Ping" uniqKey="Chen X" first="Xiu-Ping" last="Chen">Xiu-Ping Chen</name><name sortKey="Lu, Jia Hong" sort="Lu, Jia Hong" uniqKey="Lu J" first="Jia-Hong" last="Lu">Jia-Hong Lu</name><name sortKey="Lu, Jin Jian" sort="Lu, Jin Jian" uniqKey="Lu J" first="Jin-Jian" last="Lu">Jin-Jian Lu</name><name sortKey="Wang, Xiao Wen" sort="Wang, Xiao Wen" uniqKey="Wang X" first="Xiao-Wen" last="Wang">Xiao-Wen Wang</name><name sortKey="Wang, Ya Fang" sort="Wang, Ya Fang" uniqKey="Wang Y" first="Ya-Fang" last="Wang">Ya-Fang Wang</name><name sortKey="Wang, Yi Tao" sort="Wang, Yi Tao" uniqKey="Wang Y" first="Yi-Tao" last="Wang">Yi-Tao Wang</name><name sortKey="Wang, Zhao Yu" sort="Wang, Zhao Yu" uniqKey="Wang Z" first="Zhao-Yu" last="Wang">Zhao-Yu Wang</name><name sortKey="Xu, Xiao Huang" sort="Xu, Xiao Huang" uniqKey="Xu X" first="Xiao-Huang" last="Xu">Xiao-Huang Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D69 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000D69 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27184816
|texte= Induction of C/EBP homologous protein-mediated apoptosis and autophagy by licochalcone A in non-small cell lung cancer cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27184816" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |